With the increase in human population, over exploitation of bioresources and climate change, there is an ever-growing threat to biological resources and biodiversity. The ideas presented in this paper are implemented in MEGAN 4, which is available from. Hence tools are needed that allow one to analyze both types of data together, and one such tool is MEGAN. The result output is imported into MEGAN, using a synonym file that maps the SILVA accession numbers onto the NCBI taxonomy.Įnvironmental samples are often studied using both targeted 16S rRNA sequencing and random shotgun sequencing. For the analysis all 16S sequences are blasted against the SILVA database. We describe an extension of the metagenome analysis tool MEGAN, which allows one to analyze 16S sequences. Similarly, a number of tools exist for analyzing randomly sequenced DNA reads. Several tools or packages have been developed for analyzing communities using 16S rRNA sequences. There are two main approaches to metagenomics: amplicon sequencing, which involves PCR-targeted sequencing of a specific locus, often 16S rRNA, and random shotgun sequencing.

Megan's corpse falls out of the obelisk, and a keycard falls off of her.Metagenomics is a rapidly growing field of research aimed at studying assemblages of uncultured organisms using various sequencing technologies, with the hope of understanding the true diversity of microbes, their functions, cooperation and evolution. However, the results in the computer monitors start displaying 'LIVE SAMPLE REQUIRED'. After he does, the limbs fall off, and Eric takes her body and puts it into the artifact in order to resurrect Timmy. After her transformation is complete into a creature reminiscent of a Virginia, complete with popping out mutant babies, the monster attacks Eric, forcing him to eventually kill the girl. Megan then begins having a seizure, Eric steps back, and mutated foot-like appendages begin coming out her throat. Megan crashes her toy plane, then points to Eric, revealing she knows how he got to the island. As Eric gets almost within reach of the girl, Megan turns to him, revealing her mouth, hands, and feet that are all covered in blood, suggesting she is a younger cannibal now. As he gets close to her, Megan is revealed to be surrounded by drawings and a toy plane, which she 'flies' around. After Eric, the player, concludes that he needs a sacrifice in order to bring back his son, he follows her bloody footsteps around the facility, until he finds Megan in the middle of a large room. The player finds clues to her existence before meeting her through her crayon box that can be found, the several Megan Drawings, and the camcorder tapes.Įric finds a dead Mathew and Eric's own dead son shortly after finding the obelisk. However, she (presumably) killed her father upon resurrection, leaving his body nearby the obelisk, with crayons in his chest, eyes, and mouth. Mathew, her father, kidnapped Timmy at the beginning of the story to use as a sacrifice for the resurrection obelisk, which in turn worked and brought Megan back to life. After it dies, it will drop the 'cannibal' version of Megan again. The 'hands' have claws and the 'feet' have three digits. Her cannibal version warps into this, and it includes multiple deformed arms and legs. Her 'mutant' self, also known as Mutant Megan.Her hands, feet, and mouth are all covered in what appears to be blood, though it could possibly be red paint.

Her back shows vertical lacerations upon her spine, and the two sides of the cut are bridged by medical tape.

Her 'cannibal' self, after her death and resurrection.

The player can view this in the school photo, and in Camcorder tapes 1, 2, and 5. She appears to be a normal little girl with brown hair and hazel eyes, approximately the same age as Timmy.

Her 'normal' self, prior to death and mutation.

She is using what appears to be an oxygen mask and a wheelchair for her unspecified medical problems. Megan has three forms, only two of which the player can witness in person.

Megan gish forest va